rplGB [2019-06-07 17:26:33]

highly expressed protein, similar to ribosomal protein L7AE family, associated with the ribosome during exponential growth, binds K-turns in RNA switches

locus: BSU01090

pI: 9.51

mw: 8.33 kDa

protein length: 82 aa

gene length: 249 bp

function: unknown

product: unknown

essential: no

synonyms: ybaB,rpmXB,ktuS

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.4|Ribosomal proteins]

Gene

Coordinates: 129,340 129,588

The protein

Catalyzed reaction/ biological activity

• binds K-turns in [SW|RNA switch]es as they occur in the [protein|search|L-box], the [protein|search|S-box] and the [protein|search|T-box] [Pubmed|22355167]

Protein family

• Eukaryotic ribosomal protein eL8 family (with [protein|search|rplGA], according to UniProt)

Structure

• [PDB|3V7E] ([protein|11D7ABA61FC4069B48614875AF2C6C694291B545|RplGB] bound to the [S-box|search|SAM-I riboswitch] aptamer) [Pubmed|22355167]

[SW|Localization]

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]-[gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL]-[gene|8049CACE85B2627A08CB4D8A8587D52E2DB24C7F|rpsG]-[gene|297844645CCCF40CB40E84339997F6610883DF4E|fusA]-[gene|E771685D6D41D48E0C0AF77F15B4F380820FCDC2|tufA]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]
  • the mRNA is processed between [gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB] and [gene|0E4CB387626F53E5F60C7639D525B57D50C65C03|rpsL] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

Biological materials

Mutant

• BKE01090 ([gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE01090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACATATCCTCCAAAG, downstream forward: _UP4_TAACGTACTTTTGTTTTTGC
• BKK01090 ([gene|11D7ABA61FC4069B48614875AF2C6C694291B545|rplGB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK01090 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAAACATATCCTCCAAAG, downstream forward: _UP4_TAACGTACTTTTGTTTTTGC

References

• 11948165,23249744,22355167,26522935,29280348,29794222